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目的观察叔丁基过氧化氢(tBHP)对绿猴肾细胞增殖、凋亡的影响及普罗布考的保护作用。方法应用不同浓度tBHP在绿猴肾细胞建立氧化应激损伤的实验模型,采用MTT比色法检测细胞存活率、流式细胞仪分析细胞周期、Hochest染色观察细胞凋亡情况,同时观察普罗布考作用后增殖及凋亡能力的改变。结果 50μmol/L与100μmol/L tBHP作用于绿猴肾细胞24h,细胞存活率明显降低,G0-G1期细胞比例及凋亡细胞数显著增加,普罗布考可减轻上述变化。结论 tBHP能抑制绿猴肾细胞增殖并诱导凋亡,普罗布考具有保护tBHP所致损伤作用。
Objective To observe the effect of t-butyl hydroperoxide (tBHP) on the proliferation and apoptosis of green monkey kidney cells and the protective effect of probucol. Methods The experimental model of oxidative stress injury was established in green monkey kidney cells with different concentrations of tBHP. The cell viability was detected by MTT colorimetric assay. The cell cycle was analyzed by flow cytometry. Hochest staining was used to observe the cell apoptosis. Effect of proliferation and apoptosis after the change. Results After treated with 50μmol / L and 100μmol / L tBHP, the survival rate of green monkey kidney cells was significantly decreased, the percentage of cells in G0-G1 phase and the number of apoptotic cells were significantly increased, and probucol reduced the above changes. Conclusion tBHP can inhibit the proliferation of green monkey kidney cells and induce apoptosis. Probucol has the protective effect of tBHP-induced injury.