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苹浅褐卷蛾原产澳大利亚,目前仅分布于少数地区,是澳大利亚、新西兰及英国等国家多种经济作物上的重要害虫,苹浅褐卷蛾形态具有多态性,对其形态鉴定一直非常困难,由此也增加了该虫在口岸上的入侵风险。本研究使用DNA条形码技术(barcoding)测定了采集自新西兰5个不同地区苹浅褐卷蛾种群26个样本的细胞色素酶I(COI)序列。研究表明,苹浅褐卷蛾种内COI序列差异远小于3%,而与BOLD数据库中卷蛾科其他种类的种间差异约为7%～16%,远大于3%。该结果肯定了条形码技术能够成为口岸快速、准确鉴定苹浅褐卷蛾的有效方法。
Australian pink moth native to Australia, currently only distributed in a few areas, Australia, New Zealand and the United Kingdom and other countries on a variety of economic crops of the major pests, the apple moth morphological polymorphism, its morphological identification has been very Difficulties, which also increase the risk of invasion of the insects in the port. The present study used barcoding to determine the cytochrome I (COI) sequence of 26 samples collected from five populations in New Zealand. Studies have shown that the COI sequence difference of the species is much less than 3%, while the difference with the other species in the BOLD database is about 7% -16%, far greater than 3%. This result confirms that barcode technology can be an effective and effective method for rapid and accurate identification of Caenorhabditis elegans at the port.