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目的:观察血糖水平对缺氧缺血新生大鼠体质量和脑体比的影响。方法:实验于2002-10/2004-02在上海交通大学医学院附属新华医院动物实验中心完成。选择7日龄SD新生大鼠485只,随机数字表法分为10组,分别为正常组51只,不予任何处理;假手术组37只,予颈部作一切口,分离右颈总动脉后不予结扎,再缝合颈部切口;单纯低血糖组47只,于缺氧缺血前禁食12h,其后不予缺氧缺血;缺氧缺血组51只,仅给予缺氧缺血;缺氧缺血前低血糖组49只,在缺氧缺血前禁食12h后再予缺氧缺血;缺氧缺血后低血糖组44只,在缺氧缺血后禁食12h;缺氧缺血前轻度高血糖组49只,缺氧缺血后轻度高血糖组47只,分别于缺氧缺血前或后2h始皮下注射250g/L葡萄糖(1.5g/kg),共2次,每次间隔60min;缺氧缺血前重度高血糖组52只,缺氧缺血后重度高血糖组58只,分别于缺氧缺血前或后2h始皮下注射250g/L葡萄糖(1.5g/kg),共3次,每次间隔45min。后7组又分缺氧缺血后2,24,48,72h,7d5个时相点,每个时相点7～10只。于缺氧缺血即刻测定各组新生大鼠尾部血糖值,并分别在缺氧缺血即刻和各时相点称体质量,根据所测体质量和缺氧缺血即刻测得体质量之差评估各组体质量增长情况和断头后脑体比。结果:纳入动物485只,均进入结果分析。成功制备了各组模型。正常新生大鼠的体质量和脑体比随日龄增加分别呈上升和下降趋势。所有缺氧缺血组新生大鼠在缺氧缺血后24h与正常组在体质量增长和脑体比差异均呈显著性或非常显著性意义(P<0.05或0.01),尤以缺氧缺血前或后低血糖组的体质量增长最为缓慢,脑体比最高。缺氧缺血前或后合并轻或重高血糖对大鼠的体质量增长则无明显影响,缺氧缺血前重度高血糖组的脑体比与正常组比较差异无显著性意义(P>0.05)。结论:缺氧缺血合并低血糖对新生大鼠体质量增长以及脑体比的影响最为明显,缺氧缺血前或后合并高血糖对大鼠的体质量增长影响则较轻微并接近正常。
Objective: To observe the effect of blood glucose level on the body mass and brain-to-body ratio of hypoxic-ischemic neonatal rats. METHODS: The experiment was performed at the Animal Experiment Center of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from October 2002 to February 2004. A total of 485 SD neonatal 7-day-old SD rats were randomly divided into 10 groups (n = 51 in the normal group), without any treatment. 37 rabbits in the sham operation group were given an incision on the neck and the right common carotid artery No ligation, and then suture neck incision; simple hypoglycemia group 47, before hypoxia and ischemia fasting 12h, then no hypoxic-ischemic; hypoxic-ischemic group 51, only hypoxia lack Hypoxic-ischemic pre-hypoglycemia group 49, before hypoxia-ischemia for 12 hours before hypoxia-ischemia; hypoxia-ischemia hypoglycemia group 44, fasting after hypoxia-ischemia 12h ; Hypoxic-ischemic pre-mild hypoglycemia group 49, hypoxia-ischemia mild hyperglycemia group 47, respectively, before hypoxia and ischemia 2h before the subcutaneous injection of 250g / L glucose (1.5g / kg) , A total of 2 times, each interval of 60min; hypoxic-ischemic pre-severe hyperglycemia group of 52, hypoxia-ischemia group of 58 hyperglycemia, hypoxia and ischemia were 2h before the subcutaneous injection of 250g / L Glucose (1.5g / kg) for 3 times at intervals of 45min. After 7 groups were sub-hypoxic ischemia 2,24,48,72 h, 7d5 time points, each time point 7 to 10 only. Immediately after hypoxia and ischemia, the tail blood glucose of neonatal rats in each group was measured. The body weight was immediately measured at each point of hypoxia and ischemia, and the body weight was measured according to the difference between the measured body weight and the body weight measured immediately after hypoxia and ischemia The body mass growth in each group and the ratio of brain after decapitation. Results: 485 animals were included in the results analysis. Successfully prepared each model. Normal body weight and body weight of newborn rats increased with increasing age, respectively, and the downward trend. The hypoxic-ischemic neonatal rats showed significant or very significant difference in body mass gain and brain-body ratio at 24 h after hypoxia-ischemia (P <0.05 or 0.01), especially hypoxia Pre-or post-hypoglycemia group’s body mass growth is the slowest, the highest brain. Hypoxia and ischemia before or after the merger of light or hyperglycemia had no significant effect on body weight gain in rats, hypoxic-ischemic prehypertensive hyperglycemia group compared with the normal group no significant difference (P> 0.05). Conclusion: Hypoxia-ischemia combined with hypoglycemia has the most obvious effect on body weight gain and body mass ratio in neonatal rats. The hypoxia and ischemia combined with hyperglycemia on body weight gain in rats is mild and close to normal.