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用杆状病毒表达系统重组病毒，在昆虫细胞中表达了完整的含有ＥＢＶ－ＬＭＰ１基因３个外显子开放读码框架的长２．３ｋｂ的ｃＤＮＡ片段。用重组病毒感染Ｓｆ９细胞，用免疫荧光染色，结果表明：４８小时表达重组蛋白，７２小时细胞较完整，免疫荧光染色强阳性，９６小时后细胞出现破碎。我们采集７２小时的组织培养上清和细胞破碎裂解液，分别采用ＳＤＳ－ＰＡＧＥ、ＨＰＬＣ分子筛法，用免疫蛋白印迹法实验证明，表达的蛋白能被抗ＬＭＰ１的单克隆抗体所识别，测定表达蛋白的分子量为６０ｋＤ。经蛋白含量扫描图分析，采用Ｓｅｐｈａｄｅｘ－７５柱初步纯化表达的ＬＭＰ１蛋白，将后者进行裸鼠体内致瘤实验，未见肿瘤生长。
The complete 2.3 kb cDNA fragment containing the open reading frame of EBV-LMP1 gene was expressed in insect cells using recombinant baculovirus expression system. Sf9 cells were infected with the recombinant virus and stained with immunofluorescence staining. The results showed that the recombinant protein was expressed at 48 hours, more complete at 72 hours, strong positive staining by immunofluorescence, and the cells were broken after 96 hours. We collected 72 hours of tissue culture supernatant and cell lysis lysate, respectively, by SDS-PAGE, HPLC molecular sieve method, with the Western blotting experiments showed that the expression of the protein can be anti-LMP1 monoclonal antibody to determine the expression of protein Molecular weight of 60kD. The protein content of the scan map, using Sephadex-75 column preliminarily purified expression of LMP1 protein, the latter tumor in vivo nude mice experiments, no tumor growth.