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为了研究食管癌前病变组织中多种抑癌基因表达状况,我们应用P_(53)、P_(16)和nm23蛋白对74例食管癌旁异型增生组织进行了检测。结果:P_(53)和P_(16)蛋白均随异型增生病理级别的升高而标记阳性率升高,Ⅰ级异型增生P_(16)阳性率与Ⅱ～Ⅲ级比较有显著差异(P<0.005);nm23蛋白随异型增生病理级别的升高而标记阳性率下降,Ⅰ～Ⅱ级阳性率与Ⅲ级比较差异有显著性(P<0.005);P_(53)+P_(16)+nm23阳性者8例(10.8％),P_(53)+P_(16),P_(16)+nm23,P_(53)+nm23阳性者25例(33.8％),P_(53),P_(16)和nm23阳性者34例(45.9％),1种以上抑癌基因蛋白阳性者67例(90.5％)。结果提示:食管癌前病变存在P_(53)和P_(16)蛋白过度表达,P_(53)和P_(16)基因突变是食管癌发生的早期事件;nm23蛋白表达水平与异型增生病理级别呈负相关,说明nm23基因不仅能抑制肿瘤转移,而且能部分地抑制肿瘤形成;食管癌前病变存在多种抑癌基因突变或失活。
In order to study the expression of multiple tumor suppressor genes in precancerous lesions of esophageal cancer, we used P53, P16, and nm23 proteins to detect 74 cases of esophageal carcinoma atypical hyperplasia. RESULTS: The positive rates of P_(53) and P_(16) proteins increased with the increase of pathological grade of dysplasia, and the positive rate of grade I dysplasia P_(16) was significantly different from grade II~III (P< 0.005); nm23 protein decreased with the increase of the pathological grade of dysplasia and the positive rate of grade I-II compared with grade III was significant (P<0.005); P_(53)+P_(16)+nm23 8 cases (10.8%) were positive, 25 cases (33.8%) were P_(53)+P_(16), P_(16)+nm23, P_(53)+nm23 positive, P_(53), P_(16) Thirty-four (45.9%) patients were positive for nm23, and 67 (90.5%) were positive for one or more tumor suppressor protein. The results suggest that P_ (53) and P_ (16) proteins are overexpressed in precancerous lesions of esophageal cancer. P_ (53) and P_ (16) gene mutations are early events of esophageal carcinogenesis; nm23 protein expression level and pathological grade of dysplasia Negative correlation indicates that the nm23 gene not only inhibits tumor metastasis but also partially inhibits tumor formation; there are multiple tumor suppressor gene mutations or inactivation in preeclampsia.