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目的探讨S100A12在子痫前期患者母血清中的表达和临床意义。方法分别抽取24例子痫前期轻度患者、24例子痫前期重度患者以及24例正常妊娠妇女的血液,收集所有患者完整的病例资料,采用免疫印迹法检测血清中S100A12蛋白表达水平,并采双抗体夹心酶联免疫吸附法检测血清中TNF-α和IL-6的表达。结果与正常组相比,子痫前期患者血清中S100A12、IL-6和TNF-α的表达水平显著升高(P<0.05),且S100A12的表达水平与IL-6存在显著正相关性(P<0.05)。结论 S100A12在子痫前期患者血清中的表达水平明显升高,有可能用于临床上子痫患者的诊断和治疗。
Objective To investigate the expression and clinical significance of S100A12 in maternal serum of preeclampsia patients. Methods Twenty-four cases of mild preeclampsia, 24 severe preeclampsia and 24 normal pregnant women were enrolled in this study. Complete data of all cases were collected. The expression of S100A12 protein in serum was detected by Western blotting and double antibody The levels of TNF-α and IL-6 in serum were detected by sandwich enzyme-linked immunosorbent assay. Results The serum levels of S100A12, IL-6 and TNF-α in preeclampsia patients were significantly higher than those in normal controls (P <0.05), and there was a significant positive correlation between S100A12 and IL-6 <0.05). Conclusion The expression level of S100A12 in serum of patients with preeclampsia is obviously increased, which may be used in the diagnosis and treatment of clinical eclampsia.