论文部分内容阅读
应用化学分析、聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)技术和DNA测序技术,研究了西藏棕色砂壤土中微生物肥料不同施用量和施用期对青稞根际土壤理化性质和细菌群落多样性的影响。结果表明,施用谷特菌肥能显著提高土壤全氮、全磷、全钾、有机质、碱解氮、有效磷和速效钾的水平,如播前施用菌肥浓度750 ml hm-2的处理较不施用菌肥的处理上述指标分别提高13.32%、28.42%、16.20%、9.81%、21.36%、39.35%和30.48%,拔节期施用菌肥浓度2 250 ml hm-2的处理较不施用菌肥的处理分别提高7.25%、29.35%、18.04%、12.86%、15.90%、43.27%和53.99%。DGGE分析表明,相同施用方式中不同施用量土样中微生物的DGGE图谱相似。非加权组平均法(UPGMA)聚类分析将DGGE图谱分为2大类群。Shannon-Wiener指数表明,施用菌肥的土壤细菌多样性先增加后逐渐降低,播前以喷施谷特菌浓度750 ml hm-2时的细菌多样性最高;拔节期则以喷施谷特菌浓度2 250 ml hm-2处理的细菌多样性最高,且两种施用方式土壤养分的释放与Shannon指数的变化规律均为播前﹥拔节期。测序结果表明,不同施肥浓度土样微生物种群分布较为广泛,其中Actinobacteria纲细菌种类略多,少数菌种为未经培养菌种(Uncultured bacterium)。典型对应分析(CCA)表明,DGGE图谱条带分布与土壤理化性质密切相关,碱解氮、全磷和全氮是影响微生物群落的主要环境因子。研究结果表明,施用谷特菌肥可明显改善青稞根际土壤理化性状,提高土壤细菌多样性。
The effects of different application rates and application periods of microbial fertilizer on the rhizosphere soil physico-chemical properties and bacterial community in the brown sand loam of Tibet were studied by means of chemical analysis, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and DNA sequencing. The impact of diversity. The results showed that the application of Gout bacteria fertilizer could significantly increase the levels of soil total nitrogen, total phosphorus, total potassium, organic matter, available nitrogen, available phosphorus and available potassium. For example, the treatment with 750 ml hm-2 The above indexes were increased by 13.32%, 28.42%, 16.20%, 9.81%, 21.36%, 39.35% and 30.48%, respectively. The treatment with non-application of bacteriostat at the concentration of 2 250 ml hm-2 Increased by 7.25%, 29.35%, 18.04%, 12.86%, 15.90%, 43.27% and 53.99% respectively. DGGE analysis showed that the DGGE profiles of microorganisms in different application rates in the same application mode were similar. Unweighted group average method (UPGMA) cluster analysis DGGE maps are divided into two groups. The Shannon-Wiener index indicated that bacterial diversity increased firstly and then decreased with the application of bacterial fertilizer. The bacterial diversity was highest when the concentration of goutotrichum sp. 750 ml hm-2 was applied before sowing, The highest bacterial diversity was found at the concentration of 2 250 ml hm-2, and the variation of soil nutrient release and Shannon’s index were sowing time> jointing time. Sequencing results showed that the distribution of soil microorganisms in different concentrations of fertilizers was more extensive, in which Actinobacteria had slightly more bacteria species and a few species were Uncultured bacterium. Canonical correspondence analysis (CCA) showed that the distribution of DGGE bands was closely related to soil physico-chemical properties. Both alkali-hydrolyzable nitrogen, total phosphorus and total nitrogen were the major environmental factors influencing microbial community. The results show that the application of Gout bacteria fertilizer can significantly improve the rhizosphere soil of barley physicochemical properties and improve soil bacterial diversity.