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目的为适用于空间特殊实验环境,并在细胞培养实验中更高效地使用试剂、提高细胞培养用品的污染防护能力,提出了一种内部流动路径可控、具备防渗漏、防污染能力的全透明和全封闭细胞培养板。方法基于有限元仿真对培养板核心结构—流路控制挡板进行了优化,以优化后的培养板为核心部件搭建细胞培养回路,并开展了中长周期细胞培养试验。结果最优化的三坝-纵向排列细胞培养板,可有效控制其内部流体的流动路径,进出口流速1 m L/min时流体剪切力仅4.8×10~(-5)Pa,流动死区几乎为0,以该培养板为核心构建的细胞培养回路中培养的细胞可正常生长15 d。结论该细胞培养板,可使腔内新旧细胞培养试剂更换及残留气体排出更彻底,防渗漏防污染能力大大提升,试剂使用率提高,能满足空间实验常用典型细胞系的中长期培养需求,适合于我国目前及将来的空间医学生物学实验应用场合。
The purpose is to adapt to the space special experimental environment, and to use reagents more efficiently in cell culture experiments to improve the ability of cell culture supplies to protect against contamination. A new controllable internal flow path with anti-seepage and anti-pollution ability Transparent and totally enclosed cell culture plates. Methods Based on the finite element simulation, the core structure of the culture plate, the flow control baffle, was optimized. The optimized culture plate was used as the core component to build the cell culture circuit and the long-term cell culture experiment was carried out. Results The optimized three-column-lengthwise cell culture plate can effectively control the internal fluid flow path. The flow shear force is only 4.8 × 10 ~ (-5) Pa when the inlet and outlet flow rate is 1 m L / min, and the flow dead zone Almost 0, cells cultured in the cell culture circuit constructed with this plate can grow normally for 15 days. Conclusion The cell culture plate can replace the old and new cell culture reagents in the cavity and release the residual gas more thoroughly, greatly improve the anti-seepage and anti-contamination ability, increase the reagent usage rate, and meet the medium and long-term culture needs of typical cell lines commonly used in space experiments. Suitable for China’s current and future space medical biology experiments applications.