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在云南省西南边境9县市捕获伊蚊属雌性成蚊16种19367只,用细胞法和乳鼠法分离病毒。从185批6491只白纹伊蚊中分离到病毒2株,从50批1605只剌扰伊蚊中分离到病毒2株,从23批772只窄翅伊蚊中分离到病毒2株,从4批103只阿萨姆伊蚊中分离到病毒1株。其它12种共10396只伊蚊的病毒分离物为阴性。分离到的7株病毒经免疫荧光、酶免疫、血凝抑制和中和试验鉴定,均为乙型脑炎病毒(JEvirus)。白纹伊蚊是野外竹林的优势蚊种。分析认为白纹伊蚊在当地乙型脑炎病毒保存和传播中起重要作用,刺扰伊蚊、窄翅伊蚊和阿萨姆伊蚊亦可参与该病毒的传播。
19367 female mosquitoes of Aedes albopictus were captured in 9 counties and cities in the southwestern border of Yunnan Province. The viruses were isolated by the cell method and the neonatal rat method. Two strains of viruses were isolated from 185 batches of 6491 Aedes albopictus. Two strains of viruses were isolated from 50 batches of 1605 Aedes albopictus. Two strains of viruses were isolated from 23 batches of 772 Aedes albopictus, 103 batches of Assam mosquitoes isolated from a virus. The virus isolates of 10396 Aedes albopictus from other 12 species were negative. The seven viruses isolated were identified by immunofluorescence, enzyme immunoassay, hemagglutination inhibition and neutralization tests, all of which were JE virus. Aedes albopictus is the dominant bamboo species in the wild. The analysis suggested that Aedes albopictus plays an important role in the preservation and dissemination of Japanese JE virus, and that Aedes albopictus, Aedes albopictus and Aedes albopictus could also participate in the transmission of the virus.