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本文研究了α－氨基酸与水杨醛和醋酸铜定量生成席夫碱－铜（Ⅱ）配合物的最佳条件，提出了用氯仿将过量水杨醛还原的铜与席夫碱－铜（Ⅱ）配合物离心分离，然后用火焰原子吸收法在３２４．８ｕｍ处测定水相中的铜，从而间接求出α－氨基酸的含量．
In this paper, the optimal conditions for the quantitative formation of Schiff base-copper (Ⅱ) complexes of α-amino acids with salicylaldehyde and copper acetate were proposed. The results showed that the copper and the Schiff base- ) Complex was separated by centrifugation, and the copper in the aqueous phase was measured by flame atomic absorption spectrometry at 324.8 μm to indirectly determine the content of α-amino acids.