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对不同材料用蔗糖溶液预处理花药,使之产生质壁分离,再接种于不同蔗糖浓度的培养基上。探讨了玉米花药胚状体(愈伤组织)产生情况,指出胚状体(愈伤组织)与基因型关系很大,用25%浓度蔗糖预处理花药6～7min及培养基中加15%浓度蔗糖诱导频率最高。
Anther was pre-treated with different sucrose solutions to produce plasmids which were inoculated on medium with different sucrose concentrations. The embryo (callus) production of popcorn was discussed. It was found that the embryoid body (callus) had a great relationship with genotype. Pretreatment of anther with 25% sucrose for 6 ~ 7min and medium plus 15% Sucrose induction frequency of the highest.