论文部分内容阅读
利用60对SSR引物对东北52份野生大豆资源进行遗传多样性分析。结果表明:①其中40对SSR引物扩增出408个等位变异,平均每个位点等位变异10.2个,Shannon-Weaver指数变化范围为1.2203～2.6392,平均为2.0560,东北野生大豆具有较丰富的遗传变异;②41～43°N×124～126°E区的野生大豆资源分布广、遗传多样性丰富,推测处于该经纬区间的辽河平原为东北野生大豆的初生遗传多样性中心;39～41°N×120～122°E区的野生大豆遗传多样性指数较高,但搜集的资源仅有4份,推测处于该经纬区间的辽东丘陵区可能为东北野生大豆的次生多样性中心。证明东北野生大豆形成了不均衡的2个多样性中心;③基于SSR数据,把52份野生大豆材料共聚为6大类,聚类结果与野生大豆材料地理分布具有一定的相关性。
Sixty pairs of SSR primers were used to analyze the genetic diversity of 52 wild soybean resources in Northeast China. The results showed that: (1) 408 SSR primers amplified 408 alleles with an average of 10.2 alleles per locus, the Shannon-Weaver index ranged from 1.2203 to 2.6392 with an average of 2.0560, and the wild soybean in Northeast China was more abundant ; ②The wild soybean resources in the areas of 41 ~ 43 ° N × 124 ~ 126 ° E are widely distributed and rich in genetic diversity. It is speculated that the Liaohe Plain in the latitude and longitude is the center of primary genetic diversity of wild soybean in Northeast China; 39 ~ 41 The index of wild soybean in the area of ° N × 120 ~ 122 ° E was higher, but only 4 were collected. It is speculated that the Liaodong hilly area in the latitude and longitude may be the center of secondary diversity of wild soybean in northeast China. It was proved that the wild soybean in northeast China formed two unbalanced centers of diversity. (3) According to SSR data, 52 wild soybean materials were aggregated into 6 major categories, and the clustering results were related to the geographical distribution of wild soybean materials.