目的　研究以重组缺陷型腺病毒为载体的血管抑素基因治疗脑胶质瘤。方法　RT PCR法克隆angiostatin基因 ,构建携带血管抑素 (angiostatin)基因的重组腺病毒载体 ,体外检测angio statin的重组腺病毒载体对内皮细胞生长的抑制作用。建立皮下及脑胶质瘤大鼠模型 ,给予体内基因治疗。结果　克隆得到约 1 1Kb的angiostatin基因。构建重组腺病毒载体AdhCMV AGS ,体外试验表明 ,可以强烈抑制内皮细胞的生长。体内试验表明重组腺病毒可以在体内有效表达Angiostatin ,并且有效抑制胶质瘤的生长 ,使荷脑胶质瘤大鼠存活 90天以上。结论　以重组腺病毒为载体的血管抑素基因治疗脑胶质瘤效果显著 ,将是一种基因治疗的新策略 Objective To study the angiostatin gene therapy using recombinant defective adenovirus as a vector to treat glioma. Methods The angiostatin gene was cloned by RT PCR and the recombinant adenovirus vector carrying angiostatin gene was constructed. The inhibitory effect of angio-statin recombinant adenovirus vector on endothelial cell growth was assayed in vitro. Establishment of subcutaneous and glioma rat model, giving in vivo gene therapy. As a result, about 1 1 kb of angiostatin gene was cloned. Construction of recombinant adenovirus vector AdhCMV AGS, in vitro experiments showed that can strongly inhibit the growth of endothelial cells. In vivo experiments showed that the recombinant adenovirus can effectively express Angiostatin in vivo and effectively inhibit the growth of glioma and make the survival of the KLH rat more than 90 days. Conclusion The recombinant adenovirus vector for angiostatin gene therapy for glioma effect is significant, will be a new strategy for gene therapy