目的:探讨IVF-ET完全体外受精失败发生的相关危险因素。方法:回顾性分析行常规体外受精-胚胎移植(IVF-ET)的2 429个周期。应用单因素及多因素Logistic回归分析夫妇之间受孕史、精子正常形态率、精液量、精液浓度、精液活力、男方生育史、女方年龄、女方原发/继发不孕、是否有输卵管性因素、不孕年限、获卵数、月经第3日的FSH、T、PRL、LH、E2、hCG注射前LH、E2、P对完全受精失败发生的影响。结果:IVF完全不受精发生率为5.7%。完全受精失败组的精子正常形态率(11.1±5.8%)、a+b级百分比(47.4±10.5%)显著低于受精组(13.4±5.3%、50.1±8.6%)(P<0.05);完全受精失败组的女方原发不孕构成比(69.1%)、男方原发不孕构成比(74.8%)、无管性因素构成比(30.2%)、夫妇间无受孕史构成比(79.1%)显著高于受精组(36.3%、41.7%、13.6%、44.3%)(P<0.05)。此外,畸精组的完全不受精发生率(15.0%)显著高于精子形态正常组(5.2%)。Logistic回归分析亦显示精子正常形态率、精液浓度、夫妇间受孕史、不孕年限与完全受精失败有显著相关性(P<0.05)。结论:对治疗周期中畸形精子症患者、精液浓度、活力偏低、男方原发不育、女方原发不孕、无输卵管性因素、不孕年限长、夫妇之间无孕史的患者应纳入受精失败的高危人群,考虑行部分ICSI,以保障受精,对防止完全不受精的发生具有积极意义。 Objective: To investigate the related risk factors of complete in vitro fertilization failure of IVF-ET. Methods: A retrospective analysis of 2 229 cycles of routine in vitro fertilization-embryo transfer (IVF-ET) was performed. Univariate and multivariate logistic regression analysis was used to analyze the relationship between the couple’s pregnancy history, normal sperm morphology, seminal fluid volume, semen concentration, semen vitality, male reproductive history, woman’s age, female primary / secondary infertility, tubal factors , Infertility duration, number of oocytes retrieved, FSH, T, PRL, LH, E2 on the 3rd day of menstruation, LH, E2, P before hCG injection. Results: The rate of complete unfertilization of IVF was 5.7%. The percentage of normal morphology (11.1 ± 5.8%) and percentage of a + b (47.4 ± 10.5%) in the fertilization group was significantly lower than that in the fertilization group (13.4 ± 5.3%, 50.1 ± 8.6%) (P <0.05) The ratio of female primary infertility (69.1%) in male fertilization failure group, male infertility ratio (74.8%), non-sex factor constituent ratio (30.2%), non-pregnant complication ratio (79.1% (36.3%, 41.7%, 13.6%, 44.3%, respectively) (P <0.05). In addition, the rate of complete fertilization (15.0%) in the abnormal group was significantly higher than that in the normal sperm group (5.2%). Logistic regression analysis also showed that there was a significant correlation between normal sperm morphology, semen concentration, intermarine pregnancy history, infertility duration and complete fertilization failure (P <0.05). Conclusion: Patients with abnormal spermatozoa in the treatment cycle, semen concentration, low vitality, male primary infertility, the woman’s primary infertility, no tubal factors, long duration of infertility, no pregnancy history between couples should be included Infected at high risk of failure, consider the line part of the ICSI, in order to protect the fertilization, to prevent the occurrence of total fertilization has a positive meaning.