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[目的 ]调查肠出血性大肠埃希菌 O15 7∶H7在河南省 6类食品中的分布特征、污染状况及季节分布。 [方法 ]依据河南省自然地理概况分 5个采样区域 ,按夏冬季在销售环节随机取样。选择性增菌培养后 ,用 O15 7胶体金试剂筛查 ,免疫磁珠富集后分离病原菌 ,应用全自动生化鉴定系统和血清学的方法进行鉴定。 [结果 ]14 6 3份食品中共检出肠出血性大肠埃希菌 O15 7∶ H7共 2 8株 ,检出率 1.9% ,其中鲜肉和生食蔬菜检出率较高 ,分别为 3.3%和 3.2 %。夏季检出率 (2 .5 % )明显高于冬季检出率 (1.1% )。 [结论 ]肠出血性大肠埃希氏菌 O15 7∶H7在食品中污染较为严重 ,其检出率的高低与 O15 7∶ H7感染性腹泻的流行强度呈正相关。应加强畜禽屠宰、蔬菜生产和销售环节的监督监测 ,预防O15 7食源性暴发。
[Objective] To investigate the distribution characteristics, pollution status and seasonal distribution of enterohemorrhagic Escherichia coli O15 7:H7 in six kinds of food in Henan Province. [Method] According to the general situation of natural geography in Henan Province, five sampling areas were divided into sampling areas according to summer and winter seasons. After selective enrichment cultivation, O157 colloidal gold reagents were screened, and the immunomagnetic beads were collected for isolation of pathogenic bacteria. The biochemical identification system and the serological method were used to identify the pathogens. [Result] A total of 28 strains of enterohemorrhagic Escherichia coli O15 7:H7 were detected in 14 6 3 samples, with a detection rate of 1.9%. The detection rate of fresh meat and raw vegetables was 3.3% and 3.2%. The detection rate in summer (2.5%) was significantly higher than that in winter (1.1%). [Conclusion] Enterohemorrhagic Escherichia coli O15 7:H7 is more contaminated in food, and the positive rate of its detection is positively correlated with the prevalence of O15 7: H7 infectious diarrhea. Supervision and monitoring of livestock and poultry slaughtering, vegetable production and sales should be strengthened to prevent food-borne O15 outbreaks.