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为了探讨HBV与AFD_1协同致肝癌及健脾理气方防治肝癌的机理,预先予HBV转基因小鼠灌服健脾理气方2.5g/kg.wt,qd×15d,再予AFB1 1mg/kg.wt,I.P.,用RIA法测经治疗和未经治疗转基因小鼠及正常小鼠7个时相血清AFB_1—白蛋白加成物浓度。HBV转基因小鼠较正常小鼠易于聚积AFB_1毒性。高峰相加成物水平显著升高,24h相仍维持较高水平。健脾理气方能显著降低该加成物水平,在24h时相使其降至正常水平,该结果提示,HBV与AFB_1协同致肝癌是由于HBV致肝损伤,对毒性致癌物清除障碍所致,健脾理气方能通过加速毒性致癌物清除而抑制肿瘤发生。
In order to explore the mechanism of HBV and AFD_1 combined with hepatocellular carcinoma and Jianpi Liqi recipe in prevention and treatment of liver cancer, HBV transgenic mice were pre-administered 2.5 g/kg.wt, qd×15d, and then AFB1 1 mg/kg.wt. IP, serum AFB 1 - albumin adduct concentration was measured by RIA method at 7 phases of treated and untreated transgenic mice and normal mice. Compared with normal mice, HBV transgenic mice tend to accumulate AFB 1 toxicity. The level of added products at the peak was significantly increased, and the 24h phase still maintained a high level. Spleen and Qi regimen can significantly reduce the level of the adducts and reduce them to normal levels at 24 h. This result suggests that the combination of HBV and AFB_1 to cause liver cancer is caused by HBV-induced liver damage and the removal of toxic carcinogens. Spleen and Qi can inhibit tumorigenesis by accelerating the removal of toxic carcinogens.