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采用快速膜乳化法,以聚乳酸(PLA)为材料,分别以乙酸乙酯和二氯甲烷为有机溶剂,制备了粒径400和900nm的均一PLA微球和鱼精蛋白(PS)修饰的PLA-PS微球,以微球为佐剂、HBsAg为抗原免疫小鼠,在动物水平上研究其免疫调控作用,考察了不同粒径微球对抗原体内免疫效果的影响.结果表明,鱼精蛋白修饰后的PLA微球对乙肝表面抗原的吸附率增加,未修饰的PLA微球对抗原的吸附率仅为2.07%,修饰后吸附率为8.16%;修饰后微球为佐剂的免疫效果优于未修饰微球为佐剂的免疫效果,鱼精蛋白修饰能显著提升小鼠脾细胞分泌Th1型细胞因子(IL-2和IFN-γ)数(分别提升了261%和139%),分泌Th2型细胞因子IL-4数增加了67%;用修饰后平均粒径400 nm微球为佐剂免疫小鼠,IL-2分泌水平是900nm微球为佐剂的1.68倍.
A series of PLA microspheres with average diameter of 400 and 900nm and protamine (PS) -modified PLA were prepared by rapid-membrane emulsification using polylactic acid (PLA) as material and ethyl acetate and dichloromethane as organic solvents respectively. -PS microspheres with microspheres as adjuvant and HBsAg as antigen were used to immunize mice to study the immune regulation effect of the microspheres at different levels on the immune effect of the antigen.The results showed that protamine protamine The adsorption rate of modified PLA microspheres to hepatitis B surface antigen increased, the unmodified PLA microspheres adsorption rate of only 2.07%, the modified adsorption rate of 8.16%; modified microspheres adjuvant immune effect is excellent In the unmodified microspheres as an adjuvant, the effects of protamine modification on the secretion of Th1-type cytokines (IL-2 and IFN-γ) in mice spleen cells (261% and 139%, respectively) The number of Th2-type cytokines IL-4 increased by 67%; with the modified average particle size of 400 nm microspheres adjuvant-immunized mice, IL-2 secretion level is 1.68 times that of 900nm microsphere adjuvant.