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由于疟疾病人血内的疟原虫密度低,使诊断困难,也影响疫苗的生产。密度-梯度或渗透-脆性方法虽通常用以提高原虫密度,但收效不多。本文作者采用选择性高梯度磁力术分离血内的疟原虫。实验用恶性疟原虫的培养物。首次实验,在2.5×1×5.2cm的有机玻璃小室内用直径为25μm的430度不锈钢丝填充至4.6%的存储密度,放在发生0.7泰斯拉磁场的永磁极之间。小室用磷酸缓冲盐水冲洗,约10ml含有10%红细胞容积的原虫培养物(初始被原虫寄生的细胞密度为0.35%),以表
Because of the low density of malaria parasites in the blood of malaria patients, diagnosis is difficult and the production of vaccines is also affected. Although density-gradient or osmotic-fragile methods are generally used to increase protozoan density, they have not been much effective. The authors used a selective high gradient magnetic force to separate blood from Plasmodium. Experiment with P. falciparum culture. For the first experiment, a 430-degree stainless steel wire with a diameter of 25 μm was filled to a 4.6% storage density in a 2.5 × 1 × 5.2 cm plexiglass cell and placed between permanent magnetic poles where a Tesla magnetic field occurred. The chamber was rinsed with phosphate buffered saline, about 10 ml of a protozoal culture containing 10% erythrocyte volume (cell density initially parasitized by protozoa 0.35%),