以樱桃基因组DNA为模板定向克隆其ACC氧化酶基因。对PCR扩增的目的片段用 1%琼脂糖凝胶电泳检测后 ,进行回收、连续转化 ,通过蓝白筛选挑取白色菌落提取质粒DNA进行酶切鉴定 ,确定重组子后采用双脱氧终止法测定DNA全序列。DNA测序结果显示 ,樱桃的ACC氧化酶基因序列全长为 1310bp ,由 2个外显子和 3个内含子构成 ,外显子总长为 10 0 0bp。同源性分析可知 ,樱桃与桃的ACC氧化酶基因DNA序列同源性达 97 8%。 The ACC oxidase gene was cloned using cherry genomic DNA as a template. The target fragment amplified by PCR was detected by 1% agarose gel electrophoresis, then was recovered and continuously transformed. The white colonies were picked by blue-white screening to extract plasmid DNA for enzyme digestion. The recombinant was confirmed by dideoxy terminator method DNA sequence. DNA sequencing showed that the cDNA sequence of cherry ACC oxidase was 1310 bp in length, consisting of two exons and three introns, with a total length of 10 0 bp. Homology analysis showed that the DNA sequence homology of ACC oxidase gene in cherry and peach reached 97.8%.