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采用变性梯度凝胶电泳(DGGE)指纹图谱技术,对新疆乌苏泥火山区土壤细菌群落的16S rDNA V3区进行电泳分离,并用Quantity one软件对图谱进行聚类分析,对主要条带进行回收测序.DGGE图谱显示,各土样细菌的组成有显著差异.聚类分析表明,相同月份、不同深度土样细菌组成相近.特异条带的回收测序结果表明,假单胞菌属是泥火山土壤优势菌群.新疆泥火山区土壤细菌多态性明显,并容易受生态、气候等因素影响,但优势菌群受此影响较小.
The 16S rDNA V3 region of soil bacterial community in the mud volcano area of Wusu, Xinjiang was separated by electrophoresis using denaturing gradient gel electrophoresis (DGGE) fingerprinting technique. Cluster analysis was performed with the Quantity one software to recover the major bands. The results of DGGE showed that there were significant differences in the composition of bacteria in each soil samples.Cluster analysis showed that the bacterial compositions of soil samples at different depths were similar in the same month.The recovery of specific bands showed that Pseudomonas was the dominant flora of mud volcano Soil bacteria in mud volcano area of Xinjiang is obviously polymorphic and susceptible to ecological and climatic factors, but the dominant microflora is less affected.