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目的:建立血浆中尼群地平和尼莫地平对映体的毛细管电泳的手性分离方法。方法:以对硝基苯甲酸为内标,血浆样品碱化后以乙醚溶剂提取2次,运行缓冲液为40 mmol.L-1磷酸二氢钠(用磷酸调节pH至2.96)-10 mmol.L-1磺丁基醚-β-环糊精(SBE-β-CD),检测波长为237 nm,尼群地平分离电压为-18 kV,尼莫地平分离电压为-15 kV,电动进样10 s。结果:尼群地平和尼莫地平两对映体均达到基线分离,且不受杂质干扰,尼群地平和尼莫地平浓度分别在28.4～568 ng.mL-1和27.4～548 ng.mL-1范围内线性良好,最低检测浓度分别为11.4 ng.mL-1和11.0 ng.mL-1。结论:本方法快速、简单及低耗,可适用于尼群地平和尼莫地平在体内立体选择性的研究。
OBJECTIVE: To establish a chiral separation method for capillary electrophoresis of enantiomers of nitnidipine and nimodipine in plasma. Methods: Using p-nitrobenzoic acid as an internal standard, plasma samples were alkalified and extracted twice with ether solvent. The running buffer was 40 mmol·L-1 sodium phosphate monobasic (adjust pH to 2.96 with phosphoric acid) to 10 mmol. L-1 sulfobutylether-β-cyclodextrin (SBE-β-CD) at a detection wavelength of 237 nm, nitrendipine separation voltage of -18 kV and nimodipine separation voltage of -15 kV. The electrokinetic injection 10 s. Results: The enantiomers of both nitrendipine and nimodipine achieved baseline separation without interference from impurities. The concentrations of nitrendipine and nimodipine were 28.4-586 ng · mL-1 and 27.4-584 ng · mL- 1 range of linearity, the minimum detection concentrations were 11.4 ng.mL-1 and 11.0 ng.mL-1. Conclusion: The method is rapid, simple and low-cost and can be applied to study the stereoselectivity of nitrendipine and nimodipine in vivo.