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为了探索丙型肝炎病毒核心区基因疫苗对小白鼠细胞及体液免疫功能的影响，本文将HCV 核心区基因定向克隆于质粒pcDNA3 巨细胞病毒启动子下游，构建了能在真核细胞内表达的重组基因疫苗pcDNA3-HCV，将此疫苗通过多点肌肉注射免疫BALB/C 小鼠，结果发现接种了该疫苗的小鼠出现抗-HCV IgG 阳性，其抗体水平明显高于对照组，同时Th1 免疫应答相关的细胞因子IL-2 及g 干扰素水平也明显高于对照组，其结果表明，HCV 核心基因疫苗能诱导BACB/L 小鼠产生良好的细胞及体液免疫应答。
In order to explore the effect of hepatitis C core gene vaccine on the cellular and humoral immune function in mice, the core region of HCV was cloned into the downstream of the promoter of plasmid pcDNA3 cytomegalovirus in order to construct a recombinant plasmid that can be expressed in eukaryotic cells Gene vaccine pcDNA3-HCV, the vaccine by intramuscular immunization of BALB / C mice, and found that vaccinated mice showed anti-HCV IgG-positive, the antibody levels were significantly higher than the control group, while Th1 immune response Related cytokines IL-2 and g interferon levels were also significantly higher than the control group, the results showed that HCV core gene vaccine can induce good cellular and humoral immune response in BACB / L mice.