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采用氯胺丁改良法和过碘酸钠氧化法，进行抗ＡＦＰ多抗或单抗直接双标记１３１Ⅰ和ＭＭＣ，过Ｓｅｐｈａｄｅｘ－Ｇ５０柱并以ｒ计数仪与紫外分光光度仪同步监测分离、收集标记物。经测定多抗和单抗双标记物，标记比分别为７０．３ＭＢｑ∶ｍｇ∶２．２μｇ和５９．２ＭＢｑ∶ｍｇ∶１．８μｇ；放化纯度９８％和９７％；１３１Ⅰ利用率８３％和８８％；抗体回收率６２％和７４％；标记后与标记前相比抗体活性均无明显降低。置４℃５ｄ和１０ｄ时，抗体活性不受影响；放化纯度分别降为９５％和９２％、９１％和８８％。经裸鼠人肝癌模型放免显像，７２ｈ即见肿瘤区有放射性浓集，至１２０ｈ肿瘤显像最清晰。结果表明，多抗和单抗的双标记物的生物、理化性质稳定。为免疫导向治疗肝癌提供了一种新型的“双弹头”导向药物。
Using chloramine-modified method and sodium periodate oxidation method, direct anti-AFP multi-antibody or monoclonal antibody 131I and MMC were directly labeled, Sephadex-G50 column was used, and was simultaneously monitored and separated by r counter and UV spectrophotometer. Things. The multiple-antibody and monoclonal antibody dual-labelled assays have a labeling ratio of 70.3 MBq:mg:2.2 μg and 59.2 MBq:mg:1.8 μg, radiochemical purity of 98% and 97%, and 131I utilization of 83%. 88%; The antibody recoveries were 62% and 74%; there was no significant decrease in antibody activity after labeling compared to before labeling. At 4°C for 5 days and 10 days, the antibody activity was not affected; the radiochemical purity was reduced to 95% and 92%, 91%, and 88%, respectively. The radioimmunoassay of human hepatocellular carcinoma model in nude mice showed that there was radioactive concentration in the tumor area at 72 hours, and the tumor image was clearest at 120 hours. The results showed that the biological and physicochemical properties of the double-labeled multi-antibody and monoclonal antibody were stable. A new type of “double warhead”-oriented drug is provided for immune-guided treatment of liver cancer.