军团菌病首次暴发至今已有30多年历史,临床上对于军团菌的检测方法很多,细菌分离培养是军团菌检测的“金标准”。近年来,分子生物学方法检测军团菌已被越来越多的实验室所认可。本文对军团菌分子生物学检测和分型方法及其用于检测的目的基因作简要概述,并对其优劣和实用价值进行探讨。 Legionella disease for the first time since the outbreak of more than 30 years of history, clinically for Legionella detection methods are many, bacterial isolation and detection of Legionella detection “gold standard ”. In recent years, molecular biology methods to detect Legionella have been recognized by more and more laboratories. In this paper, the method of molecular biology detection and typing of Legionella and its target genes for detection are summarized briefly, and its merits and practical values ​​are discussed.