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为了研究滇重楼花药开闭运动的影响因子及细胞形态学调控机制,观察花药开闭运动现象与对应生态因子变化,设计不同光照、温度、湿度试验和人工淋雨试验,观察各因子对花药开闭运动的影响,制作石蜡切片,观察花药开闭运动时细胞形态变化。结果表明花药早开晚闭运动由外界生态因子调节,总体趋势是高温、强光、低湿利于花药开裂,低温、弱光、高湿利于花药闭合。该实验中,各生态因子对花药开裂的影响从大到小依次为湿度、温度、光照,各生态因子对花药闭合的影响从大到小依次为光照、湿度、温度,雨天雨滴的直接接触导致花药的快速闭合。从其细胞形态结构变化看,花药早开晚闭运动就是一个花粉囊壁细胞失水开裂和吸水闭合的过程,推测不同光照、温度和湿度条件引发了细胞内不同的生理活动,导致细胞渗透压变化,进而导致花药开裂或闭合。而花药遇雨闭合是一个简单的物理调节过程,雨点落在花药上,直接浸泡花药,导致花药细胞渗透压变化吸水闭合。
In order to study the influence factors of anther opening and closing movement and cell morphology regulation mechanism in Yunnan Zhongzhu Lou, observe the changes of anther opening and closing movement and corresponding ecological factors, and design different light, temperature, humidity test and artificial rain test, The effect of opening and closing movement, making paraffin sections, observation of anther opening and closing movement of cell morphology changes. The results showed that anther early open late closed movement was regulated by external ecological factors. The general trend was high temperature, high light and low humidity, which was conducive to anther dehiscence. Low temperature, low light and high humidity were favorable for anther closure. In this experiment, the influence of various ecological factors on the anther cracking was humidity, temperature and light. The ecological factors’ influence on anther closure was descending order: light, humidity, temperature, direct contact of raindrops Anthers quickly closed. From the morphological changes of the cells, the early anthesis opacification is a process of watermelon cytoplasmic cell dehiscence and water absorption closure. It is speculated that different light, temperature and humidity conditions trigger different physiological activities in the cell, leading to cell osmotic pressure Change, which in turn causes the anther to crack or close. The anther rain closure is a simple physical adjustment process, the raindrops fall on the anthers, direct soaking anthers, resulting in changes in osmotic pressure of the anther cells to close the water absorption.