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以5～7d龄的 丽春 番茄无菌苗子叶作为外植体,研究了子叶外植体对抗生素卡那霉素的敏感性,抗生素卡那霉素对番茄筛选的适宜浓度为70mg/L.通过根癌农杆菌 Agrobacteriumtumefaciens 介导,成功地进行了马槟榔甜蛋白基因MBLII对番茄的遗传转化,获得转化番茄抗性植株,组织化学法有阳性表现、PCR特异扩增及Southern杂交检测出现特异条带,表明MBLII基因已顺利整合到转基因番茄植株的基因组.
The germination rate of konjac cotyledon explant of kudzuvine was studied by using as explants the sterile seedling leaves of 5 to 7 days old callus. The optimum concentration of antibiotic kanamycin for tomato screening was 70mg / L. Through the Agrobacterium tumefaciens mediated Agrobacterium tumefaciens, the genetic transformation of tomato is successfully carried out by using MBLII gene of horse betel nut protein, and the tomato plants transformed with tomato were obtained. The positive results of histochemical method were confirmed by PCR, and the specific bands Band, indicating that the MBLII gene has been successfully integrated into the genome of transgenic tomato plants.