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目的探讨慢性前列腺炎患者前列腺液中TNF-α、IL-10和IL-2的表达在慢性前列腺炎中发病的作用,评估这些细胞因子的变化与慢性前列腺炎分类、症状和前列腺液白细胞计数的关系。方法通过尿常规、前列腺液常规检查、两杯法尿液细菌培养和NIH-CPSI评分,将58例前列腺炎患者按NIH前列腺炎诊断标准进行分型。采用双抗体夹心ELISA法检测患者及12例正常对照组前列腺液中的IL-2、TNF-α、IL-10的含量。用SPSS for Windows 12.0统计软件对测量数据进行统计学分析。结果对照组、Ⅱ型、Ⅲa型及Ⅲb型CPPS患者EPS中TNF-α水平分别为(17.8±9.2)pg/ml、(91.5±34.8)pg/ml、(74.9±35.1)pg/ml、(23.9±12.9)pg/ml,IL-10水平分别为(234.9±56.6)pg/ml、(392.7±62.4)pg/ml、(364.1±77.1)pg/ml、(300.4±80.2)pg/ml,IL-2水平分别为(311.4±67.5)pg/ml、(247.7±96.1)pg/ml、(278.2±77.6)pg/ml、(299.7±78.6)pg/ml。Ⅱ型、Ⅲa型患者EPS中TNF-α和IL-10水平与对照组及Ⅲb型比较差异均有统计学意义(P<0.05),各型CP患者EPS中IL-2水平均低于对照组,但Ⅱ型与对照组比较差别有统计学意义(P<0.05)。TNF-α含量和白细胞计数呈正相关性(r=0.772,P<0.01),而IL-10含量与患者疼痛评分呈正相关(r=0.549,P<0.01)。结论慢性前列腺炎患者EPS中TNF-α、IL-10和IL-2表达变化,提示它们参与了前列腺的炎症反应。TNF-α、IL-10有可能成为CP诊断分型的一个依据,而IL-10水平有可能作为患者疗效评估的一个指标。
Objective To investigate the role of TNF-α, IL-10 and IL-2 expression in prostatic fluid in patients with chronic prostatitis in the pathogenesis of chronic prostatitis and to evaluate the relationship between these cytokines and the classification, symptoms and prostatic fluid white blood cell count relationship. Methods 58 cases of prostatitis were classified according to the diagnostic criteria of NIH prostatitis by routine urine test, prostatic fluid routine examination, urine culture by two cups method and NIH-CPSI score. The levels of IL-2, TNF-α and IL-10 in prostatic fluid of patients and 12 normal controls were detected by double antibody sandwich ELISA. The SPSS for Windows 12.0 statistical software for statistical analysis of measurement data. Results In control group, the levels of TNF-α in EPS of type Ⅱ, type Ⅲa and type Ⅲ b CPPS patients were (17.8 ± 9.2) pg / ml, (91.5 ± 34.8) pg / ml and 23.9 ± 12.9 pg / ml and IL-10 levels were (234.9 ± 56.6) pg / ml, 392.7 ± 62.4 pg / ml, 364.1 ± 77.1 pg / ml and 300.4 ± 80.2 pg / ml, IL-2 levels were (311.4 ± 67.5) pg / ml, (247.7 ± 96.1) pg / ml, (278.2 ± 77.6) pg / ml and (299.7 ± 78.6) pg / ml respectively. The levels of TNF-α and IL-10 in type Ⅱ and type Ⅲa patients were significantly lower than those in control group and type Ⅲb (P <0.05). The levels of IL-2 in EPS of EPS group were lower than those in control group , But the difference between type Ⅱ and control group was statistically significant (P <0.05). There was a positive correlation between the level of TNF-α and leukocyte count (r = 0.772, P <0.01), while the level of IL-10 was positively correlated with the pain score (r = 0.549, P <0.01). Conclusion The expression of TNF-α, IL-10 and IL-2 in EPS of chronic prostatitis patients suggest that they are involved in the inflammatory response of the prostate. TNF-α, IL-10 may become a basis for the diagnosis of CP, and IL-10 levels may be used as an indicator of the efficacy of the patient evaluation.