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以筛选出的5份优异草木樨(Melilotus)种质PI595335、MO-LX04、PI595388、Ames19257和PI593234为材料,分别用不同渗透势的PEG(0、-0.3、-0.6、-0.9、~(-1).2、~(-1).5 MPa)及不同浓度的NaCl溶液(0、50、100、150、200、300mmol·L~(-1))模拟干旱和盐碱胁迫条件,采用纸上发芽法进行种子萌发试验,测定发芽率、发芽势、胚根幼苗长、发芽指数、活力指数等指标,并综合比较不同种质的抗旱及耐盐能力。结果表明,1)高渗透势(≥-0.3 MPa)的条件能不同程度地促进5份种质的萌发,对PI595335、MO-LX04、PI595388胚根和幼苗的生长有一定抑制作用,对Ames19257、PI593234胚根和幼苗的生长有显著的(P<0.05)促进作用;而较低渗透势(≤-0.9 MPa)则显著抑制(P<0.05)所有种子的萌发与幼苗生长。2)不同浓度的NaCl溶液对5份种质草木樨幼苗的生长均有显著的(P<0.05)抑制作用,而低浓度的NaCl(≤100mmol·L~(-1))溶液能促进草木樨种子的萌发。3)用模糊数学隶属法综合评价结果显示,5份草木樨种质在种子萌发期抗旱性强弱为Ames19257>PI593234>MO-LX04>PI595335>PI595388,耐盐性强弱为PI593234>Ames19257>PI595388>MO-LX04>PI595335。其中Ames19257和PI593234在抗旱性和耐盐模拟试验中的表现均明显优于其它3份草木樨种质,更适于为今后杂交创制新种质提供遗传材料或在西北干旱、盐碱地区种植。
Five of the elite Melilotus germplasms, PI595335, MO-LX04, PI595388, Ames19257 and PI593234, were screened with PEG (0, -0.3, -0.6, -0.9, 1) .2, -1 (-1) .5 MPa) and different concentrations of NaCl solution (0, 50, 100, 150, 200 and 300 mmol·L -1) were used to simulate the drought and saline- Germinating method was used to test the seed germination. The germination rate, germination potential, radicle seedling length, germination index and vitality index were measured. The drought resistance and salt tolerance of different germplasms were compared. The results showed that 1) the condition of high osmotic potential (≥-0.3 MPa) could promote the germination of 5 germplasms to some extent, and inhibited the growth of radicle and seedlings of PI595335, MO-LX04 and PI595388. Ames19257, PI593234 had a significant (P <0.05) promoting effect on the growth of radicle and seedling, while lower osmotic pressure (≤-0.9 MPa) significantly inhibited (P <0.05) germination and seedling growth of all seed. 2) Different concentrations of NaCl solution had significant (P <0.05) inhibitory effect on the growth of five seedlings of G. bracteatum, while low concentrations of NaCl (≤100 mmol·L -1) Seed germination. 3) Fuzzy mathematics subordination method comprehensive evaluation showed that the germination of five germplasm resources germplasm Ames19257> PI593234> MO-LX04> PI595335> PI595388, the salt tolerance of PI593234> Ames19257> PI595388 > MO-LX04> PI595335. Among them, Ames19257 and PI593234 exhibited significantly better drought resistance and salt tolerance than those of the other three plants, and were more suitable for providing genetic material for future new germplasm development in hybridization or planting in arid and saline-alkali areas of Northwest China.