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目的:探讨风湿性心脏病(风心病)慢性心力衰竭时心肌细胞内肌浆网Ca2+转运功能变化的机制。方法:采用Westernblot法测定19例风心病患者(风心病组)和6例意外脑死亡者(对照组)心肌肌浆网钙泵蛋白和兰尼碱受体含量,同时采用草酸盐易化的肌浆网Ca2+摄取法测定两组心肌肌浆网的C...
Objective: To investigate the mechanism of Ca2 + transport in sarcoplasmic reticulum (Ca2 +) in cardiomyocytes in patients with rheumatic heart disease (rheumatic heart disease) complicated with chronic heart failure. Methods: Western blotting was used to determine the content of cardiac muscle sarcoplasmic reticulum calcium receptor and the content of ryanodine receptor in 19 patients with rheumatic heart disease (rheumatic heart disease) and 6 patients with unexpected brain death (control group). Meanwhile, oxalate facilitated muscle Pulp Ca2 + uptake method for the determination of two groups of myocardial sarcoplasmic reticulum C ...