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目的:探讨H2O2是否通过调节P38MAPK的活性而诱导PC12细胞凋亡。方法:碘化丙啶(PI)染色流式细胞术(FCM)检测细胞凋亡;Western-Blot测定磷酸化ERK1/2蛋白和磷酸化p38蛋白的表达。结果:作用PC12细胞24h后,20～80μmol/L的H2O2可呈浓度依赖性地诱导PC12细胞凋亡并增加PC12细胞P38磷酸化的水平;P38特异性抑制剂SB203580(10或20μmol/L)预处理30min可显著减轻40μmol/LH2O2对PC12细胞凋亡的诱导作用。结论:H2O2通过上调P38的活性而诱导PC12细胞凋亡。
AIM: To investigate whether H2O2 induces PC12 cell apoptosis by regulating the activity of P38MAPK. Methods: Apoptosis was detected by flow cytometry (FCM) with propidium iodide (PI) staining. The expression of phosphorylated ERK1 / 2 and phosphorylated p38 protein was detected by Western-Blot. RESULTS: After treated with PC12 cells for 24 h, 20 ~ 80 μmol / L H2O2 could induce the apoptosis of PC12 cells in a concentration-dependent manner and increase the phosphorylation of P38 in PC12 cells. P38-specific inhibitor SB203580 (10 or 20 μmol / L) Treatment with 30 min could significantly reduce the induction of PC12 cell apoptosis by 40 μmol / LH 2 O 2. Conclusion: H2O2 induces PC12 cell apoptosis by up-regulating the activity of P38.