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目的探讨乙醇脱氢酶2(ADH2)基因的Arg47His变异与慢性乙型肝炎(慢乙肝)之间的关系。方法采用住院病例对照研究设计。以252例中国汉族慢乙肝患者为慢乙肝组,以223名中国汉族正常人为对照组。PCR-RFLP方法检测ADH2基因的Arg47His变异。运用F检验比较各组之间的均数差异;运用χ2分析各组之间的频数分布差异;运用单因素及多因素Logistic回归分析ADH2基因Arg47His变异与慢性肝炎之间的关系。结果 ADH2基因Arg47His变异Arg与His等位基因频率在慢乙肝组(37.3%、62.7%)与对照组(28.5%、71.5%)比较,差异有统计学意义(P=0.005);Arg/Arg、Arg/His、His/His基因型频率在慢乙肝组(13.1%、48.4%、38.5%)与对照组(7.2%、42.6%、50.2%)比较,差异有统计学意义(P=0.014)。以His/His基因型为参照组,Arg/His与Arg/Arg基因型单因素Logistic回归分析OR分别为1.48(95%CI:1.01～2.17)与2.38(95%CI:1.24～4.59)。调整性别、年龄、吸烟、饮酒、肝炎家族史后,其OR分别为1.46(95%CI:0.97～2.19)与2.51(95%CI:1.25～5.02)。结论 ADH2基因Arg47His变异野生型基因类型可能是中国汉族人群慢性乙型肝炎发病的重要危险因素之一。
Objective To investigate the relationship between Arg47His mutation of alcohol dehydrogenase 2 (ADH2) gene and chronic hepatitis B (chronic hepatitis B). Methods In-hospital case-control study design. A total of 252 Chinese Han patients with chronic hepatitis B were chronic hepatitis B patients and 223 Chinese Han normal subjects as control group. PCR-RFLP method to detect Arg47His variation of ADH2 gene. F-test was used to compare the mean difference between each group. Χ2 was used to analyze the frequency distribution differences among groups. Univariate and multivariate Logistic regression analysis was used to analyze the relationship between Arg47His variation of ADH2 gene and chronic hepatitis. Results The Arg and His allele frequencies of Arg47His mutation in ADH2 gene were significantly different between the two groups (37.3%, 62.7%) and the control group (28.5%, 71.5%) (P = 0.005) The frequencies of Arg / His and His / His genotypes in the chronic hepatitis B group (13.1%, 48.4%, 38.5%) were significantly different from those in the control group (7.2%, 42.6%, 50.2%) (P = 0.014). Using the His / His genotype as reference group, the logistic regression analysis of Arg / His and Arg / Arg genotypes were 1.48 (95% CI: 1.01-2.17) and 2.38 (95% CI: 1.24-4.59) respectively. The odds ratio (OR) was 1.46 (95% CI: 0.97-2.19) and 2.51 (95% CI: 1.25-5.02) after adjustment for gender, age, smoking, drinking, and family history of hepatitis. Conclusion The genotypes of wild-type Arg47His gene of ADH2 gene may be one of the important risk factors of chronic hepatitis B in Chinese Han population.