戊型肝炎是一种严重危害人类健康的疾病 ,因其致病因子戊型肝炎病毒 (HEV)的细胞培养一直不很成功 ,影响了戊肝研究的深入。学者们在这方面进行了大量研究 ,尝试了各种不同来源的细胞和不同的培养条件 ,积累了很多经验 ,其中一些细胞系已试试用于 HEV的检测和疫苗的筛选。本文就 HEV细胞培养方面的进展与应用作一介绍。 Hepatitis E is a serious disease that is endangering human health because the cell culture of hepatitis E virus (HEV), a causative agent of the disease, has been unsuccessful, affecting the further study of HepG. Scholars have done a lot of research in this area, tried a variety of different sources of cells and different culture conditions, has accumulated a lot of experience, some of which have been tested for HEV cell lines and vaccine screening. This article gives an introduction to the progress and application of HEV cell culture.