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AIM:To study the serum concentration of IL-1β,IL-1receptor antagonist(IL-1Ra)and IL-18 in Gαi2-deficientmice at the age of 6(healthy),12(pre-colitic)and 24 wk(colitic)and in healthy control mice.METHODS:At the time of killing,serum sampleswere collected and IL-1β,IL-1Ra and IL-18 levels weremeasured using enzyme-linked immunosorbent assays.RESULTS:Serum concentration of IL-1Ra wassignifcantly increased in pre-colitic(median:524 ng/L;P=0.02)and colitic(450 ng/L;P=0.01),but not inhealthy(196 ng/L)Gαi2-deficient mice as compared withcontrols(217 ng/L).Serum concentrations of IL-1β didnot differ between Gαi2-deficient mice and their controls,irrespective of age,IL-18 was significantly increased incolitic,but not in pre-colitic mice compared with controls(510 ng/L vs 190 ng/L;P=0.05).CONCLUSION:The increased serum concentrations ofIL-18 and IL-1Ra in established diseases are suggestedas markers of ongoing colitis.Interestingly,thesignificantly increased serum concentration of IL-1Ra inpre-colitic mice is found to be an early marker of diseaseprogression.
AIM: To study the serum concentration of IL-1β, IL-1 receptor antagonist (IL-1Ra) and IL-18 in Gαi2-deficient mouse at the age of 6 (healthy), 12 (pre-colitic) and 24 wk and in healthy control mice. METHODS: At the time of killing, serum sampleswere collected and IL-1β, IL-1Ra and IL-18 levels weremeasured using enzyme-linked immunosorbent assays.RESULTS: Serum concentration of IL-1Ra wassignifcantly increased in pre -colitic (median: 524 ng / L; P = 0.02) and colitic (450 ng / L; P = 0.01) but not in Healthy (196 ng / L) Gαi2- deficient mice as compared with controls (217 ng / L). Serum concentrations of IL-1β did not differ between Gαi2-deficient mice and their controls, irrespective of age, IL-18 was significantly increased incolitic, but not in pre-colitic mice compared with controls (510 ng / L vs 190 ng / L; P = 0.05) .CONCLUSION: The increased serum concentrations of IL-18 and IL-1Ra in established diseases are suggested as markers of ongoing colitis. Indeed, the latter clearly increased serum concentration of IL-1Ra i npre-colitic mice is found to be an early marker of diseaseprogression.