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目的观察由Raw264.7细胞诱导破骨细胞形成过程中黑皮质素受体(MCR)的作用。方法用α-促黑素(α-MSH)及其类似物SHU9119、PT141、THIQ分别处理体外培养的Raw 264.7细胞,并依此分为对照组、α-MSH组、SHU9119组、PT141组、THIQ组和α-MSH加SHU9119组。培养6天后,经抗酒石酸酸性磷酸酶染色后,观察并计数各组破骨细胞形成数目。RT-PCR法测定Raw 264.7细胞表达的MCR种类。结果在Raw 264.7细胞诱导破骨细胞过程中,α-MSH呈剂量依赖性的增加了破骨细胞形成。SHU9119组、PT141组及α-MSH加SHU9119组均显著增加了破骨细胞形成数目(P<0.05);但THIQ处理组对破骨细胞形成无统计学意义(P>0.05)。Raw 264.7细胞表达5种MCR。结论 MCR激动剂能显著增加破骨细胞形成数目,可能主要通过结合MCR1和/或MCR5促进破骨细胞形成。
Objective To observe the effect of melanocortin receptor (MCR) induced by Raw264.7 cells during osteoclastogenesis. Methods Raw 264.7 cells were treated with α-melanin (α-MSH) and its analogues SHU9119, PT141 and THIQ respectively and divided into control group, α-MSH group, SHU9119 group, PT141 group, THIQ Group and α-MSH plus SHU9119 group. After cultured for 6 days, the number of osteoclast formation in each group was observed and counted after stained with tartrate-resistant acid phosphatase. The RT-PCR method was used to determine the type of MCR expressed by Raw 264.7 cells. Results α-MSH increased osteoclasts in a dose-dependent manner during the induction of osteoclasts by Raw 264.7 cells. The numbers of osteoclasts were significantly increased in SHU9119, PT141 and α-MSH plus SHU9119 groups (P <0.05). However, the formation of osteoclasts in THIQ group was not statistically significant (P> 0.05). Raw 264.7 cells express 5 MCRs. Conclusion MCR agonists can significantly increase the number of osteoclasts, which may promote the formation of osteoclasts mainly through the binding of MCR1 and / or MCR5.