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[目的]评价小麦TaPIMP1基因在小麦耐旱种质培育方面的应用潜力。[方法]将由ubiquitin启动子驱动的TaPIMP1转基因纯合株系B64和B208及受体对照扬麦158在模拟的干旱胁迫条件下进行培养,测定种子萌发、幼苗生长以及部分耐旱相关生化指标的变化。[结果]在20%PEG6000模拟干旱胁迫条件下,转基因株系及其对照的TaPIMP1表达量在24 h内变化剧烈;转基因株系的种子发芽率、胚芽鞘和胚根长度显著高于受体对照。在10%~20%PEG6000模拟干旱胁迫条件下,转基因株系的叶片相对含水量和可溶性糖含量明显高于受体对照。在15%~20%PEG6000模拟干旱胁迫条件下,转基因株系的叶片丙二醛(MDA)含量明显低于受体对照。[结论]该研究表明转基因株系的耐旱能力较受体对照扬麦158有显著提高,TaPIMP1基因可应用于转基因小麦耐旱种质的培育。
[Objective] The research aimed to evaluate the potential of wheat TaPIMP1 gene in cultivating drought-tolerant wheat germplasms. [Method] Transgenic homozygous TaPIMP1 transgenic lines B64 and B208 driven by ubiquitin promoter and Yangmai 158, a receptor control, were cultured under simulated drought stress to determine the changes of seed germination, seedling growth and some drought-related biochemical indexes . [Result] Under the conditions of 20% PEG6000 simulated drought stress, the expression level of TaPIMP1 in transgenic lines and its control changed drastically within 24 h. The seed germination rate, coleoptile and radicle length of transgenic lines were significantly higher than those of control . Under the conditions of 10% ~ 20% PEG6000 simulated drought stress, the relative water content and soluble sugar content of the transgenic lines were significantly higher than that of the receptor control. Under 15% ~ 20% PEG6000 simulated drought stress, MDA content in transgenic lines was significantly lower than that in recipient controls. [Conclusion] This study showed that the drought tolerant ability of the transgenic lines was significantly higher than that of the recipient Yangmai 158, and TaPIMP1 gene could be used to cultivate the drought-tolerant germplasm of transgenic wheat.