Objective: To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission electron microscopy (TEM), genome extraction and nucleases test were used to isolate bacteriophages. Then its morphology and characteristics were examined. Results: A novel bacteriophage XY-1 was isolated from a sewage pond in a hospital. It infected and killed 6 E. coli reference strains. The phage had a round head (diameter 40-50 nm), no tail and the genome was ssRNA of approximately 5. 0 kb. It was able to reduce E. coli to an extent of 44. 63% to 67. 00% when being added into the samples of different raw sewage water, depending on the contact time, the temperature and the phage dose. Conclusion; From the morphology typical and nucleotide characteristics (RNA) of the genome of phage, phage XY-1 appears to be closely related to phage f2. It may have some effects for the control of E. coli in sewage water.