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目的观察氟哌啶醇对豚鼠单个心室肌细胞延迟整流钾电流(IK)通道的作用,探讨氟哌啶醇致心律失常的离子基础。方法应用全细胞膜片钳技术记录氟哌啶醇对豚鼠单个心室肌细胞IK的影响。结果在获得单个细胞后,分别给予0.3、1.0、3.0μmol/L的氟哌啶醇。延迟整流钾电流在试验电压+70 mV时,从给药前的(16.660 4±1.571 7)分别减少至(5.025 5±1.937 2)、(9.857 2±3.900 2)、(7.179 4±1.816 0)pA/pF(n=7,P<0.01)。结论氟哌啶醇可抑制IK,使动作电位时程延长而引发心律失常,其致心律失常作用可能与其对钾通道的作用有关。
Objective To observe the effect of haloperidol on delayed rectifier potassium current (IK) channel in single ventricular myocytes of guinea pig and to investigate the ionic basis of haloperidol induced arrhythmia. Methods Whole-cell patch-clamp technique was used to record the effect of haloperidol on IK in isolated guinea pig ventricular myocytes. Results After single cells were obtained, 0.3, 1.0 and 3.0 μmol / L of haloperidol were given respectively. The delayed rectifier potassium current decreased from (16.660 4 ± 1.571 7) to (5.025 5 ± 1.937 2), (9.857 2 ± 3.900 2) and (7.179 4 ± 1.816 0), respectively, at the test voltage of +70 mV pA / pF (n = 7, P <0.01). Conclusions Haloperidol can inhibit IK, prolong the action potential duration and cause arrhythmia. Its arrhythmia may be related to the effect of potassium channel.