农吉利(Crotalaria sessiliflora L.)为豆科野百合属植物,民间用于治疗肿瘤。药用部分为全草,有效成分为生物碱,种子中含量最高,其中农吉利碱(monocrotaline)经动物试验有明显的抗肿瘤作用。农吉利碱为双稠吡咯啶类生物碱,具有内酯环。因此试验了与羟胺及三氯化铁的反应,得红色溶液,但空白太高。又试验了 Polonovsky 反应,利用农吉利碱结构中的3-吡咯啉环,用过氧化氢将其氧化成 N-氧化物,加乙酸酐加热后去氢成吡咯,与 Ehrlich 试剂(4-二甲氨基苯甲醛)产生有色物。此方法虽然很 The Crotalaria sessiliflora L. is a plant belonging to the genus Lilium in the leguminous family and is used for the treatment of tumors. The medicinal part is whole plant, the active ingredient is alkaloid, and the content of seeds is the highest. Among them, monocrotaline has obvious anti-tumor effect through animal experiments. Agropurine is a dipyridyl pyrrolidine alkaloid with a lactone ring. Therefore, the reaction with hydroxylamine and ferric chloride was tested to give a red solution, but the blank was too high. The Polonovsky reaction was tested again, using the 3-pyrroline ring in the agropurine structure, oxidizing it with hydrogen peroxide to N-oxides, heating with acetic anhydride to dehydrogenate to pyrrole, and Ehrlich reagent (4-dimethylbenzene) Aminobenzaldehyde) produces a colored product. This method is very