论文部分内容阅读
通过液体培养方法研究补骨脂提取物对苹果腐烂病菌菌丝生长的抑制作用,同时探究补骨脂提取物对病原菌菌丝体内几丁质酶及-β1,3葡聚糖酶活性的影响。结果表明:当补骨脂提取物浓度为5,10,20,40,80 mg·L-1时,对苹果腐烂病菌菌丝干重的抑制率分别为39.8%,62.3%,79.4%,90.6%,98.3%;用50mg.L-1补骨脂提取物处理在液体培养3d的苹果腐烂病菌,处理后1,3,6,12h菌体内几丁质酶活性分别为对照的1.32,3.62,3.34,2.47倍;β-1,3葡聚糖酶活性分别为对照的4.78,9.98,5.83,3.53倍。由此表明,补骨脂提取物对苹果腐烂病菌具有明显的抑制作用。
The inhibitory effect of psoralen extract on the mycelial growth of apple rot pathogen was studied by liquid culture method. Meanwhile, the effect of psoralen extract on the activities of chitinase and -β1,3-glucanase in mycelium of pathogens was also explored. The results showed that when the concentration of Psoralen extract was 5, 10, 20, 40 and 80 mg · L-1, the inhibitory rates of mycelial dry weight of apple rot disease were 39.8%, 62.3%, 79.4% and 90.6% % And 98.3%, respectively. After treated with 50 mg.L-1 of psoralen for three days, the chitinase activities in the liquid of 1, 3, 6 and 12 h were 1.32 and 3.62 respectively, 3.34,2.47 times; β-1,3-glucanase activity were 4.78,9.98,5.83,3.53 times the control. This shows that psoralen extract has obvious inhibitory effect on apple rot pathogen.