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研究了红缘拟层孔菌固体发酵产物对H22荷瘤小鼠的抗肿瘤和抗氧化作用,以抑瘤率、脾和胸腺指数、白细胞介素-2、干扰素-r、血管内皮细胞生长因子、超氧化物歧化酶、丙二醛、过氧化氢酶、谷胱甘肽过氧化物等指标来考察红缘拟层孔菌固体发酵产物对H22荷瘤小鼠肿瘤抑制和体内抗氧化作用。结果表明,固体发酵产物高剂量和中剂量组的抑瘤率分别为66.66%和64.70%,与阴性组比较,有显著的抗肿瘤作用(P<0.01),HE染色切片也能观察到固体发酵产物高、中和低各组肿瘤细胞大量坏死,并且高剂量和低剂量组血清中白细胞介素-2和干扰素-r含量显著增加,血管内皮生长因子含量降低,与抑制肿瘤效果具有一定相关性;此外,红缘拟层孔菌固体发酵产物各组可以降低丙二醛含量,提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物含量;综上所述,红缘拟层孔菌固体发酵产物具有显著的抗肿瘤和抗氧化作用。
The anti-tumor and anti-oxidative effects of the solid Fermentation product of Rhodosporidium aquatica on H22 tumor-bearing mice were studied. The anti-tumor rate, spleen and thymus index, interleukin-2, interferon-r and the growth of vascular endothelial cells Factors, superoxide dismutase, malondialdehyde, catalase, glutathione peroxidase and other indicators to investigate the fermentation of Pinacea pseudotuberculosis solid H22 tumor-bearing mice tumor inhibition and in vivo anti-oxidation . The results showed that the inhibition rates of solid fermentation products were 66.66% and 64.70%, respectively. Compared with the negative control group, the antitumor activity was significant (P <0.01). Solid fermentation was also observed by HE staining The product of high, medium and low tumor necrosis in each group, and high-dose and low-dose group serum interleukin -2 and interferon-r content was significantly increased vascular endothelial growth factor levels, and the inhibition of tumor effect has a certain correlation In addition, each group can reduce the content of malondialdehyde, increase superoxide dismutase, catalase, glutathione peroxide content; In summary, the Red Edge Pseudotubacteria solid fermentation products have significant anti-tumor and anti-oxidation.