论文部分内容阅读
目的检测分析自来水管网生物膜中细菌的类群及其可能对人类健康的威胁,为自来水管网生物膜的卫生学评价提供参考。方法截取某市政自来水管网实际管段,冲刷收集管壁生物膜,共采集10个生物膜样品,参照GB/T5750.12—2006检验方法测定细菌总数和大肠菌群,参照2010年版中国药典关于纯化水的微生物检测方法测定贫养细菌,使用流式细胞法检测活菌数目,用志贺菌显色培养基、沙门菌显色培养基、BCYE培养基和TCBS培养基对志贺菌、沙门菌、军团菌和霍乱弧菌分离鉴定并计数。结果 10个生物膜样品中,营养琼脂法检测细菌数目为101cfu/cm2~104cfu/cm~2,R2A琼脂培养法细菌计数为102cfu/cm~2~106cfu/cm~2,流式细胞法检测的活菌数目为104cfu/cm~2~108cfu/cm~2。大肠菌群检出率达80.0%,志贺菌检出率为60.0%,沙门菌检出率为50.0%,未检出军团菌和霍乱弧菌。结论自来水管网生物膜存在大量细菌,自来水管网生物膜对人体健康存在潜在威胁。
Objective To detect and analyze the bacterial groups in the biofilm of tap water pipelines and their potential threat to human health, and to provide reference for the hygienic evaluation of biofilm in tap water pipelines. Methods The actual pipe section of a municipal water pipe network was intercepted and scoured to collect the biofilm of the pipe wall. A total of 10 biofilm samples were collected. The total bacterial count and coliforms were determined according to GB / T5750.12-2006 test method, referring to the 2010 Chinese Pharmacopoeia The microorganism detection method of water was used to determine the number of bacteria in poor condition. The number of viable cells was determined by flow cytometry. The growth of Shigella spp. And Salmonella spp. Were detected by using Shigella colorimetric medium, Salmonella chromogenic medium, BCYE medium and TCBS medium. Legionella and Vibrio cholera were identified and counted. Results The number of bacteria detected by nutrient agar was 101cfu / cm2 ~ 104cfu / cm2 in 10 biofilm samples, while the bacterial counts in R2A agar culture method were 102cfu / cm2 ~ 106cfu / cm2. The flow cytometry The number of viable cells is 104 cfu / cm ~ 2 ~ 108 cfu / cm ~ 2. Coliform group detection rate was 80.0%, Shigella detection rate was 60.0%, Salmonella detection rate was 50.0%, did not detect Legionella and Vibrio cholerae. Conclusion There are a lot of bacteria in the biofilm of tap water pipe network, and the biofilm of tap water pipe network poses a potential threat to human health.