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目的 :探讨喉癌基因 c- erb B- 2点突变及过表达同喉癌发生发展的关系。方法 :应用 PCR- SSCP对c- erb B- 2的点突变进行检测 ,采用免疫组化法分析 c- erb B- 2的过表达。结果 :40例喉癌阳性表达率为 52 .5%( 2 1 / 4 0 )。其中 2 4例声门上癌表达率为 57% ( 1 7/ 30 ) ;6例声带癌表达率为 50 % ( 3/ 6) ,4例声门下癌表达率为 2 2 .5% ( 1 / 4 ) ,同前者差异有高度显著性 ( P<0 .0 1 ) ,未见 c- erb B- 2的点突变检出。结论 :喉癌中广泛存在着 c- erb B- 2的扩增及过表达 ,而喉癌细胞中却无 c- erb B- 2点突变 ,推测可能存在着另外的基因改变或 c-erb B- 2的扩增和过表达为喉癌发生的主要原因。
Objective: To investigate the relationship between the mutation of c-erb B-2 and the overexpression of laryngeal carcinoma and the occurrence and development of laryngeal carcinoma. Methods: The point mutation of c-erb B-2 was detected by PCR-SSCP and the overexpression of c-erb B-2 was analyzed by immunohistochemistry. Results: The positive rate of 40 cases of laryngeal carcinoma was 52.5% (2 1/40). Among them, the expression rate of supraglottic carcinoma was 57% (17/30) in 24 cases, 50% (3/6) in 6 cases of vocal cord cancer, and 22.5% (1 / 4), with the former significant difference (P <0. 01), no c-erb B-2 point mutation detected. CONCLUSION: There is widespread amplification and overexpression of c-erb B-2 in laryngeal carcinoma, but no c-erb B-2 mutation in laryngeal cancer cells, suggesting that there might be other gene changes or c-erb B - 2 amplification and overexpression as the main reason for the occurrence of laryngeal cancer.