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为延长硫酸链霉素的作用时间,提高其对烟草青枯病的抗病能力并减少其残留,利用壳聚糖与γ-聚谷氨酸间的静电自组装作用形成纳米微胶囊,在成囊过程中将硫酸链霉素负载其中,制备了壳聚糖/γ-聚谷氨酸负载硫酸链霉素纳米微胶囊,并对不同硫酸链霉素用量及壳聚糖和γ-聚谷氨酸的质量比对成囊效果及负载效果的影响进行了研究。结果表明:随着硫酸链霉素添加量的增加,微胶囊的载药量增大;随着壳聚糖与γ-聚谷氨酸质量比的减小,不同硫酸链霉素添加量下,包封率均呈增大趋势,同时载药量均呈降低趋势;当硫酸链霉素添加量为10 mg,m(壳聚糖)∶m(γ-聚谷氨酸)=20∶17时,包封率最高(85.2%),载药量达46.8%。傅立叶变换红外光谱分析及差示扫描量热法分析结果表明,微胶囊对硫酸链霉素负载效果较好;透射电子显微镜下,载药纳米微胶囊呈规整实心球形,粒径分布较均匀,平均粒径在20～50 nm之间;模拟释放结果表明,纳米微胶囊对硫酸链霉素具有缓释效果。
In order to prolong the action time of streptomycin sulfate, enhance its resistance to tobacco bacterial wilt and reduce its residual, nanocapsules were formed by electrostatic self-assembly between chitosan and γ-polyglutamic acid. Streptomycin sulphate was loaded into the vesicles and chitosan / γ-polyglutamic acid loaded streptomycin nanocapsules were prepared, and the amount of streptomycin sulfate and chitosan and γ-polyglutamine The effect of acid mass ratio on encapsulation efficiency and loading effect was studied. The results showed that with the increase of the amount of streptomycin sulfate, the drug loading of the microcapsules increased. With the decrease of mass ratio of chitosan to γ-polyglutamic acid and the amount of streptomycin added, The entrapment efficiency showed an increasing trend, while drug loading showed a downward trend; when the streptomycin sulfate addition amount of 10 mg, m (chitosan): m (γ-polyglutamic acid) = 20:17 , The highest encapsulation efficiency (85.2%), drug loading capacity of 46.8%. Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analysis showed that microcapsules had a better loading effect on streptomycin sulfate; under the transmission electron microscope, the drug-loaded nanocapsules were uniformly spherical with a uniform particle size distribution, The particle size ranged from 20 to 50 nm. The simulated release results showed that the nanocapsules had a sustained release effect on streptomycin sulfate.