为探讨龙牙楤木总皂苷(AS)对缺血/再灌注(I/R)诱导大鼠心肌细胞损伤的保护作用,采用乳酸钠灌注液灌注成年大鼠的心室肌细胞模拟缺血,含钙台式液模拟再灌注,细胞收缩与离子浓度同步测定系统同步检测AS对单个I/R细胞收缩和钙瞬变的影响。发现AS使再灌注后心肌细胞静息态肌小节长度、收缩幅度、收缩/舒张速度以及钙瞬变幅度、速度增大(P<0.05,P<0.01),细胞达到舒张时最大长度的90.0%的时间、细胞收缩达峰值的时间、稳态钙静息值、收缩期[Ca2+]i上升到最高的50.0%的时间、胞内钙瞬变衰减率减少(P<0.05,P<0.01)。因此,推测AS改善了缺血再灌注后细胞收缩与钙稳态的损伤。 To investigate the protective effect of Aralia elata (SA) on myocardial cell injury induced by ischemia / reperfusion (I / R) in rats, the ventricular myocytes of adult rats were infused with sodium lactate perfusion solution to simulate ischemia, Simultaneous determination of cell contraction and ion concentration by benchtop liquid simulated reperfusion, cell contraction and ion concentration simultaneously detected the effect of AS on contraction and calcium transient of single I / R cells. It was found that the AS length, systolic, systolic / diastolic velocities and the amplitude and velocity of resting calcium in cardiomyocytes after reperfusion were increased (P <0.05, P <0.01) (P <0.05, P <0.01). The time of peak of cell contraction, the steady-state calcium resting value, and the systolic [Ca2 +] i up to 50.0% of the time were decreased. Therefore, it is hypothesized that AS improves cell contraction and calcium homeostasis after ischemia-reperfusion.