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将乙脑P3株鼠脑毒种在Vero细胞中适应性传代，当传至10代时，毒种的CPE和感染性虽无明显变化，但其免疫原性却明显减弱，因此，用在Vero细胞中传递1至2代的几株建立毒种库（P3V1和P3V2），其涵度分别为7．71和78210gpfu／ml。在毒种中加入以山梨醇为主要成分的稳定剂后，4～S℃保存30天，－30℃1年，－60℃2年，滴度降低约0．51ogpfu／ml，在生产中使用效果良好。
The virulence of P3 virulence strain of JE-3 was passaged in Vero cells adaptively. However, the CPE and infectivity of the virulent strain did not change obviously when it reached passage 10, but its immunogenicity was obviously weakened. Therefore, Several strains of cells that passed from passage 1 to passage 2 established pools of poisons (P3V1 and P3V2) with a degree of concordance of 7.71 and 78,210 gpfu / ml, respectively. After the stabilizer containing sorbitol as the main component is added to the virus species, the product is stored for 4 days at 30 ° C for 30 days, at 1 year at 30 ° C, for 2 years at 60 ° C, and the titer is reduced by about 0.51 μg pfu / ml for use in production Good effect.