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目的:建立UPLC同时测定桂枝中原儿茶酸、香豆素、肉桂醇、肉桂酸和桂皮醛的含量。方法:采用ACQUITYUPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),以乙腈-0.05%的磷酸水溶液为流动相,梯度洗脱,流速0.5 mL.min-1,检测波长254 nm,柱温30℃。结果:原儿茶酸、香豆素、肉桂醇、肉桂酸和桂皮醛分别在0.359～3.59 mg.L-1(r=0.999 3),2.83 4～28.34 mg.L-1(r=0.999 8),0.574～5.74 mg.L-1(r=0.999 8),2.400～24.00 mg.L-1(r=0.999 9),和32.57～325.7 mg.L-1(r=0.999 8)(n=6)有良好的线性关系;平均加样回收率(n=9)均在96.7%～101.0%,RSD均小于2.3%。结论:该方法快速、准确、重现性好,可作为测定桂枝中原儿茶酸、香豆素、肉桂醇、肉桂酸和桂皮醛含量的方法。
Objective: To establish a UPLC method for the simultaneous determination of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamaldehyde in Guizhi. METHODS: ACQUITY UPLC, HSS T3 column (2.1 mm × 100 mm, 1.8 μm) was used with mobile phase of acetonitrile-0.05% phosphoric acid as mobile phase at a flow rate of 0.5 mL · min-1. The detection wavelength was 254 nm. Temperature 30 ℃. Results: The concentrations of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamaldehyde ranged from 0.359 to 3.59 mg · L -1 (r = 0.999 3) and 2.83 4 ~ 28.34 mg · L -1 (r = 0.999 8 ), 0.574-5.74 mg.L-1 (r = 0.999 8), 2.400-24.00 mg.L-1 (r = 0.999 9) and 32.57-325.7 mg.L-1 (r = 0.999 8) 6) had a good linear relationship. The average recoveries (n = 9) ranged from 96.7% to 101.0% with RSDs less than 2.3%. Conclusion: The method is rapid, accurate and reproducible. It can be used as a method to determine the content of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamic aldehyde in Guizhi.