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在30只隔离灌流颈动脉窦区的麻醉大鼠,观察了辣椒素(capsaicin,CAP)对颈动脉窦压力感受性反射的影响。结果显示(1)以CAP(1 μmol/L)隔离灌流大鼠左侧颈动脉窦区时,颈动脉窦压力感受性机能曲线向左下方移位,曲线最大斜率(peak slope,PS)由0.34±0.01增至0.42±0.01(P<0.01),反射性血压下降幅度(reflex decrease,RD)由36.51±1.26增至45.01±0.71 mmHg(尸<0.01)。阈压、平衡压和饱和压分别从70.43±2.09、95.5±1.71和177.60±1.37 mmHg下降至52.86±2.80、87.00±1.58、163.55±2.12 mmHg(P<0.01)。其中Ps和RD的变化呈明显的剂量依赖性。(2)用香草酸受体亚型(vanilloid receptor subtype 1,VR1)阻断剂钌红(ruthenium red,100 μmol/L)预处理后,CAP的上述反射效应即被阻断。(3)先给予KATP通道阻断剂格列苯脲(glibenclamide,20μmol/L)也取消了CAP对压力感受性反射的影响。结果表明,CAP对大鼠颈动脉窦压力感受性反射有易化作用,此作用似与VR.介导的KATP通道开放有关。
The effect of capsaicin (CAP) on the carotid sinus baroreflex was observed in 30 anesthetized rats with isolated perfused carotid sinus. The results showed that (1) When the left carotid sinus was isolated by CAP (1 μmol / L), the carotid sinus pressure-sensitive functional shift shifted to the lower left. The peak slope (PS) of the curve was increased from 0.34 ± 0.01 to 0.42 ± 0.01 (P <0.01). The reflex decrease (RD) increased from 36.51 ± 1.26 to 45.01 ± 0.71 mmHg (P <0.01). Threshold, equilibrium pressure and saturation pressure decreased from 70.43 ± 2.09, 95.5 ± 1.71 and 177.60 ± 1.37 mmHg to 52.86 ± 2.80, 87.00 ± 1.58 and 163.55 ± 2.12 mmHg, respectively (P <0.01). Among them, the changes of Ps and RD were significantly dose-dependent. (2) The above reflex effect of CAP was blocked by pretreatment with vanilloid receptor subtype 1 (VR1) inhibitor ruthenium red (100 μmol / L). (3) The first KATP channel blocker glibenclamide (20μmol / L) also abolished the impact of CAP on baroreflex. The results show that CAP can ease the carotid sinus baroreflex in rats, which may be related to the VR-mediated KATP channel opening.