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目的建立测定血清Ⅰ型前胶原(procollagen typeⅠ,PCⅠ)放射免疫分析法(放免法),应用于前列腺癌骨转移的诊断。方法从羊胎皮中提取PCⅠ免疫兔获取特异性抗血清。结果经聚丙烯酰胺凝胶电泳证实为单一区带,β疏基乙醇还原后,出现I型前胶原特有的链。用此抗原免疫兔获得高效价抗血清,效价为1∶128倍和1∶64倍以上。结论抗原、抗体可用于PCⅠ放免法的建立。
Objective To establish a method for the determination of serum procollagen type Ⅰ (PCⅠ) by radioimmunoassay (RIA), which is used in the diagnosis of bone metastasis of prostate cancer. Methods Extract PC Ⅰ immune rabbit from sheep skin to obtain specific antiserum. The results confirmed by polyacrylamide gel electrophoresis as a single zone, β-mercaptoethanol reduced type I procollagen-specific chain. The antigens were used to immunize rabbits to obtain high titer antiserum with titers of 1:128 times and 1:64 times more. Conclusion Antigens and antibodies can be used to establish PC Ⅰ radioimmunoassay.