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目的:测定黄连根中小檗碱含量对不同UV-B辐射强度的响应,为提高黄连小檗碱含量提供理论参考。方法:实验设置了CK(自然光),UL(0.05 W.m-2),UM(0.10 W.m-2),UH(0.20 W.m-2)4个处理组,探讨UV-B辐射下初生代谢中光合特性、PPP途径和次生代谢中酪氨酸酶及黄连根中的小檗碱含量的变化特性。结果:在UH辐射胁迫下,黄连叶片中光合色素、PSII非光化学猝灭系数(qN),初始荧光(Fo),电子传递速率(ETR)以及6-磷酸葡萄糖脱氢酶活性、根中小檗碱含量均较其他组低。而处于UM辐射下的黄连,光合作用能力、PPP途径、酪氨酸酶活性以及小檗碱含量均高于对照组,产生了胁迫应激反应来抵御适度的UV-B辐射。结论:黄连处于UM辐射时,通过增强光合作用及PPP途径,能提供较多的次生代谢物前体物及次生代谢物所必需的NADPH,进而小檗碱的含量也相应提高,为黄连栽培种植中提高小檗碱含量提供参考。
Objective: To determine the content of berberine in Coptis chinesis to different UV-B radiation intensity response, to provide a theoretical reference to improve berberine content of berberine. Methods: Four treatment groups, CK (natural light), UL (0.05 Wm-2), UM (0.10 Wm-2) and UH (0.20 Wm-2) were set up to study the photosynthetic characteristics of primary metabolism under UV- Changes of Tyrosinase and Berberine Content in Coptis Root in PPP Pathway and Secondary Metabolism. Results: Under UV radiation stress, photosynthetic pigments, non-photochemical quenching coefficient (qN), initial fluorescence (Fo), electron transport rate (ETR) and glucose-6-phosphate dehydrogenase activity in Coptis chinensis leaves, Content lower than other groups. Coptis, photosynthetic capacity, PPP pathway, tyrosinase activity and berberine content under UM radiation were higher than the control group, resulting in a stress response to moderate moderate UV-B radiation. CONCLUSION: Coptis chinensis could provide more NADPH, which is necessary for secondary metabolite precursors and secondary metabolites, by enhancing photosynthesis and PPP pathway while radix UM, and the content of berberine is also correspondingly increased Cultivation planting to improve the content of berberine provide a reference.