论文部分内容阅读
目的 探讨宫内发育迟缓 (IU GR)时胰岛素样生长因子 受体 (IGF- R)表达的变化及其在组织和细胞中的定位。方法 将 2 1只 SD孕鼠随机分为实验组 12只和对照组 9只。钳夹实验组孕鼠双侧子宫动、静脉 2 0分钟 ,建立宫内发育迟缓动物模型。对照组仅作开腹和关腹术。两组均于孕 2 2天剖宫取出胎鼠 ,采用免疫组化法观察孕晚期胎鼠肝脏和肺组织中 IGF- R的表达。结果 实验组胎鼠的体重、身长及胎盘、肝、肺组织重量较对照组明显降低 (P<0 .0 5 ) ;实验组胎鼠肝脏 IGF- R表达面积比较对照组明显增加 ,平均灰度明显降低 (P<0 .0 1) ;实验组胎鼠肺组织 IGF- R表达面积比也较对照组明显增加 (P<0 .0 5 ) ,但平均灰度则无显著性差异 (P>0 .0 5 )。结论 宫内发育迟缓时 IGF- R在肝脏和肺组织的表达增加 ,可能是机体对 IGF- 降低的代偿机制
Objective To investigate the expression of insulin-like growth factor receptor (IGF-R) in intrauterine growth retardation (IU GR) and its localization in tissues and cells. Methods Twenty-one SD pregnant rats were randomly divided into experimental group (n = 12) and control group (n = 9). The experimental group of pregnant rats were intrauterine growth retardation animal models of intrauterine growth retardation after establishment of bilateral uterine artery and vein 20 minutes. Control group only for laparotomy and abdominal surgery. Fetal rats were removed from the cesarean section in gestational day 22, and the expression of IGF-R in the liver and lung tissues of the third trimester of pregnant women was observed by immunohistochemistry. Results The body weight, body length, weight of placenta, liver and lung in experimental group were significantly lower than those in control group (P <0.05). The area of liver IGF-R expression in experimental group was significantly increased compared with control group, (P <0.01). The area ratio of IGF-R expression in the lungs of experimental rats was significantly higher than that of the control group (P <0.05), but there was no significant difference between the average gray scales (P> 0 .0 5). Conclusions The intrauterine growth retardation IGF-R expression in the liver and lung tissue increased, may be the body’s compensatory mechanism of IGF-lowering